Rapid communication: mapping of the beta Tropomyosin (TPM2) gene to pig chromosome 1.
نویسندگان
چکیده
Locus. Pig Tropomyosin beta (TPM2) gene. Genus and Species. Sus Scrofa. Source and Description of Primers. Primers were derived by using well-conserved regions of published human and mouse sequence (GenBank accession nos. AF209746 and NM_009416). The primers were used to amplify a 1.4-kp fragment of the porcine TPM2 gene covering exons 3 through 8. The porcine cDNA sequence (GenBank accession no. AF420022) showed 95% exonic identity (325 bp) at the nucleotide level to the human skeletal muscle beta tropomyosin (GenBank accession no. X06825). Additionally, pig specific primers were designed to amplify a 246-bp piece that spans exons 3 to 4 (GenBank accession # AF420023) to be used for linkage and physical mapping.
منابع مشابه
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عنوان ژورنال:
- Journal of animal science
دوره 80 5 شماره
صفحات -
تاریخ انتشار 2002